Author: Sheillah Ayiela Orono, George Chege Gitao, Jean Pierre Mpatswenumugabo, Maurine Chepkwony, Christine Mutisya, Edward Okoth, Barend Mark de Clare Bronsvoort, George Cameron Russell, Vishvanath Nene and Elizabeth Anne Jessie Cook
Year: 2019
About this Publication:
A retrospective study of WA-MCF in cattle was conducted at Kapiti Plains Ranch Ltd., Kenya. During this period, 325 animals showed clinical signs of WA-MCF and of these, 123 were opportunistically sampled. In addition, 51 clinically healthy animals were sampled. Nested polymerase chain reaction (PCR) and indirect enzyme linked immunosorbent assay (ELISA) were used to confirm clinically diagnosed cases of WA-MCF. A latent class model (LCM) was used to evaluate the diagnostic parameters of clinical diagnosis and the tests in the absence of a gold standard. Clinical diagnosis was demonstrated to be an effective method to identify affected animals although animals may be incorrectly classified resulting in financial loss. The study revealed indirect ELISA as a poor test and nested PCR to be a more appropriate confirmatory test for diagnosing acute WA-MCF. However, the logistics of PCR make it unsuitable for field diagnosis of WA-MCF. The future of WA-MCF diagnosis should be aimed at development of penside techniques, which will allow for fast detection in the field.
Grant: PLSHL2
Subject Areas: Research and Development
URL https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-019-1818-8
Keywords:
ELISA, Herpesvirus, MCF, No-gold standard, PCR, Wildebeest malignant catarrhal fever
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